Mochy is a diagnostic testing laboratory is to identify the
presence of bacteria in urine
(bacteriuria). urine culture done to isolate and identify pathogenic microorganism
(s) responsible for causing urinary tract infection (UTI). urinary tract infection is more common in women
and in children than in adult males. UTI associated with
discomfort (usually burning) when urinating, and may be accompanied by
fever, malaise, and at the bottom abdominal or back pain
. All urinary structures other than the urethra, usually
sterile. Most of the organisms reach the bladder, ureters and kidneys
ascending the urethra.
The most commonly encountered urinary tract is the causative agent
E.coli. Enterococcus faecal is the most common gram-positive organisms >> << cause UTI. Infections
Klebsiella, Proteus,
, and other Enterobacteriaceae are common. Some organisms> ;> << less frequently encountered, such as
Candida Albicans,
Haemophilus influenzae, Mycobacterium tuberculosis, salmonella
SPP., and
Staphylococcus aureus usually enter the urinary system through the blood or lymph vessels. There are several different methods used to collect urine >> << sample for culture. most common is the crossing clean catch
technology. should wash their hands before starting work. Women
The external genitalia
washed two or three times with detergent and rinse with water >>. << In men, the external head of the penis the same
cleaned and rinsed. Then the patient is instructed to start piss
, and urine collected in a sterile container crossing >>. << In infants, the urinary collection bag (a plastic bag with
glue printing one end) attached to the labia girl or boy penis
to collect samples. Another method of catheterization of urine, which >> << lubricated catheter (thin rubber tube) is inserted through in
bladder. This avoids contamination from the urethra or external> > << genitals. If the patient is already a urinary catheter in place
urine can be collected zatyskayuchy tube below >> << port collection and use of sterile needles and syringes for
urine, urine can not be taken from the urinal, and >> << is not fresh and had the opportunity for growth of bacteria at room temperature
. In rare cases, your doctor may collect urine >> < <sample by inserting a needle directly into the bladder (suprapubic aspiration
). bladder puncture warranted when repeated attempts to culture >> << increase urine contamination from the urethra. It
particularly common in children. Nadlobkovaya Crane also noted when
anaerobic UTI is suspected. urine should be cultured for one hour if the collection no
cooling. However, refrigerated samples can store up
24 hours before coating sample. urine are quantitative >> << procedure. calibrated inoculation loop that contains 0. 01 or 0. 001 ml
inserted vertically in the urine sample of urine and use
pass urine in a sterile agar plate. If the urine turns
puncture the bladder, 0. 1 ml is transferred to the plate using a sterile pipette
. urine evenly across the plate with
, glass rod, unlike bands plate with a loop.
This procedure is usually performed on 5% agar plates of sheep blood
that detects the growth of most organisms, and the disc McConkie
agar or other selective and differential medium for isolation

Gram-negative microorganisms. In addition, certain urine laboratory plate
kolistyn agar-nalidixic acid (CNA) or other selective medium for
Gram-positive bacteria. plane incubated at 36 `C for 18
24 hours and read for growth. number of colonies multiplied
on the appropriate factor to give the number of colonies per ml urine. Some organisms such as Mycobacterium tuberculosis
,
can be isolated from
urine and require special nutrient media and growth conditions
. plates that show no growth at 24 h incubation another day
and read again. growth of more than three types of points
contamination and culture should be repeated with the new
sample. from one to three types of plates and made part
identification (eg, gram-negative rod, lactose positive) is
reported when there is less than 10,000 colony units (CFU) per ml
. Usually when less than 10,000 CFU / ml strattera cost recovered
body is contaminant from the urethra.
Exceptions are the availability
Staphylococcus aureus and organisms
isolated from the sample catheter or suprapubic aspiration. General >> << urethral contaminants include coagulase staphylococci,
diptheroids and lactobaccilli. Each colony type gives 10,000 or more
CFU / ml identified and antibiotic sensitivity testing
done. UTI is diagnosed when a species produces more >> << 100 000 CFU / ml. counts between 10,000 and 100,000 can be
significant, depending on the organism and the particular patient
conditions (for example, urine collected from the catheter or patient >> << receiving antibiotic treatment. to drink a glass of water 15-20 minutes before the test
useful if there is no urge to urinate. There are no other specific drugs or postoperative need
for testing. There are no risks associated with the culture test yourself.
If you insert the urinary catheter is required for urine
is a small risk of introduction of infection from the catheter. Patients who received antibiotic treatment before collection can
lead to negative effects of culture. urine is usually sterile and should not be any growth.
More than 100 000 CFU / ml of any one type of colony is
evidence IMP. Any growth on the catheter or suprapubic specimen or
growth in Staphylococcus aureus
considered significant. more
10 000 CFU / ml be significant in some populations patients and
clinical conditions. patient collects his own sample using
instructions the doctor or nurse.
Clinical laboratory scientist, NCA (CLS) / medical technologist, MT (ASCP)
usually performing culture and sensitivity testing. >> << doctor diagnoses and treatment decisions based on colony
graph organism (s) determined the antibiotic susceptibility profile
Analysis of urine and the patient specific conclusions. bacteriuria it is the presence of bacteria in the urine. << >>